N-Acetyl-β-D-Glucosaminidase (NAG)

半岛bd体育手机客户端 说明

一般描述

N-acetyl-β-D-glucosaminidase (NAG) is mainly used in the colorimetric assay for the determination ofN-acetyl-β-D-glucosaminidase in urine. NAG hydrolyzes 3-cresolsulfonphthaleinyl-N-acetyl-D-glucosaminide to release 3-cresolsulfonphthalein, which is measured photometrically at 580nm. It is an intracellular lysosomal enzyme, present in the S3 segment of proximal tubular cells and distal nephron. Presence of NAG in the urine shows an organelle impairment in proximal tubule. Level of NAG rises in individuals over 70 years of age.

应用

N-acetyl-β-D-glucosaminidase (NAG) has been used for preparing standards for the determination ofN-acetyl-β-D-glucosaminidase in urine in life-science research applications.

包装

1 kit containing 3 components

制备说明

Working solution:Preparation of solutions:

1. Buffer solution
   Dissolve the contents of bottle 1 with 55 ml double-distilledwater.
2. Substrate solution
   Dissolve the contents of bottle 2 with 55 ml solution I.
3. Stop reagent
   Dissolve the contents of bottle 3 with 110 ml double-distilled water.

Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 ℃, protected from light.
Solution III is stable for 1 month stored at 2 to 8 ℃.

Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 ℃ and for one month when stored at -15 to -25 ℃.

其他说明

For life science research only. Not for use in diagnostic procedures.

半岛bd体育手机客户端 性质

用途	sufficient for ~50 tests
质量水平	100
manufacturer/tradename	Roche
运输	ambient
储存温度	room temp

组份列表

组份不可单独销售

货号	组份
	Buffer Substance consisting of citric acid and potassium citrate
	Lyophilized Substrate consisting of sodium 3-cresolsulfonphthaleinyl-N-acetyl-β-D-glucosaminide and borax
	Stop Reagent consisting of sodium carbonate

安全信息

象形图
警示用语：	Danger
危险声明	H319 - H360FD
预防措施声明	P201 - P202 - P280 - P308 + P313 - P337 + P313 - P501
危险分类	Eye Irrit. 2 - Repr. 1B
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK	WGK 2
闪点(F)	does not flash
闪点(C)	does not flash