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MISSION?Lenti microRNA, Human

品牌
Sigma-Aldrich
货号
HLMIR1825
包装型号
规格纯度
hsa-miR-4757-5p
价格
7541.62 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
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半岛bd体育手机客户端 名称:
MISSION ?Lenti microRNA, Human
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Sigma′s Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.

Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.

其他说明

Based on miRBase V20 Mature ID

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Two negative controls are available: NCLMIR001 and NCLMIR002

法律信息

MISSION is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRES NA.51

半岛bd体育手机客户端 性质

质量水平 200
半岛bd体育手机客户端 线 MISSION?
浓度 ≥1x106VP/ml (via p24 assay)
成熟序列 AGGCCUCUGUGACGUCACGGUGU
Sanger mature/minor 登记号 MIMAT0019901
Sanger microRNA登记号 MI0017398
运输 dry ice
储存温度 ?70℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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