MISSION^?Lenti microRNA, Human

半岛bd体育手机客户端 说明

一般描述

Sigma′s Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.

Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.

其他说明

Based on miRBase V20 Mature ID

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Two negative controls are available: NCLMIR001 and NCLMIR002

法律信息

MISSION is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.51

半岛bd体育手机客户端 性质

质量水平	200
半岛bd体育手机客户端 线	MISSION?
浓度	≥1x106VP/ml (via p24 assay)
成熟序列	UCGAGGAGCUCACAGUCUAGU
Sanger mature/minor 登记号	MIMAT0004697
Sanger microRNA登记号	MI0000809
运输	dry ice
储存温度	?70℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable