浜у搧璇存槑
涓€鑸弿杩?/p>
Sigma鈥瞫 Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.
Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.
鍏朵粬璇存槑
Based on miRBase V20 Mature ID
鎺ㄨ崘浜у搧
Two negative controls are available: NCLMIR001 and NCLMIR002
娉曞緥淇℃伅
MISSION is a registered trademark of sigma-aldrich Co. LLC
鍩烘湰淇℃伅
| NACRES | NA.51 |
浜у搧鎬ц川
| 璐ㄩ噺姘村钩 | 200 |
| 浜у搧绾军/td> | MISSION? |
| 娴撳害 | ≥1x106VP/ml (via p24 assay) |
| 鎴愮啛搴忓垪 | UUCUCGAGGAAAGAAGCACUUUC |
| Sanger mature/minor 鐧昏鍙饵/td> | MIMAT0004770 |
| Sanger microRNA鐧昏鍙饵/td> | MI0003180 |
| 杩愯緭 | dry ice |
| 鍌ㄥ瓨娓╁害 | ?70鈩傸/td> |
瀹夊叏淇℃伅
| 鍌ㄥ瓨鍒嗙被浠ｇ爜 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闂偣(F) | Not applicable |
| 闂偣(C) | Not applicable |
m.cnreagent.com
