MISSION^?Lenti microRNA Inhibitor, Human

半岛bd体育手机客户端 说明

一般描述

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. The lentiviral microRNA Inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) is included, allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells.

• Allows for potent inhibition of the desired miRNA
• Lentiviral delivery format allows for efficient delivery of the inhibitor into a wide variety of cell types
• Enables long-term inhibition without repeat transfection

其他说明

Based on miRBase V19 Mature ID

法律信息

MISSION is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.51

半岛bd体育手机客户端 性质

质量水平	200
半岛bd体育手机客户端 线	MISSION?
浓度	≥1x106VP/ml (via p24 assay)
technique(s)	capture ELISA: 106TU/mL using p24 (Volume 200 uL)
成熟序列	GUUCUCCCAACGUAAGCCCAGC
Sanger mature/minor 登记号	MIMAT0003298
Sanger microRNA登记号	MI0003643
运输	dry ice
储存温度	?70℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable