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位置: 首页> 品牌> Sigma-Aldrich> Lenti CRISPR Human HPRT1 Positive Control Plasmid DNA (LV04 vector)

Lenti CRISPR Human HPRT1 Positive Control Plasmid DNA (LV04 vector)

品牌
Sigma-Aldrich
货号
CRISPR21
包装型号
规格纯度
价格
2486 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Lenti CRISPR Human HPRT1 Positive Control Plasmid DNA (LV04 vector)
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

The Lenti CRISPR Human HPRT1 Positive Control Plasmid DNA (LV04 vector) is a critical positive control to monitor transfection efficiency for lentiCRISPRs. The protein encoded by this gene is a transferase which plays a central role in the generation of purine nucleotides. Cells with intact HPRT1 will die upon exposure to 6-TG (Product No. A4882), an antimetabolite used in the treatment of leukemias. Conversely, cells with HPRT1 knocked out will increase over time with 6-TG selection. This targets a validated HPRT1 CRISPR site, which serves as an experimental control for lenti CRISPRs. The lenti CRISPR positive control is a dual-component system consisting of a U6-driven guide RNA plasmid targeting the human HPRT1 gene and hPGK-driven Puromycin and BFP expression. This control requires Cas9 be delivered by a separate vector.

应用

功能性基因组学研究/筛选/靶标验证

其他说明

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).

法律信息

CRISPR Use License Agreement

Lentiviral License Agreement

基本信息

NACRES NA.51

半岛bd体育手机客户端 性质

质量水平 200
包装 vial of 50 μL
浓度 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s) CRISPR
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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