KAPA SYBR^?FAST

半岛bd体育手机客户端 说明

一般描述

KAPA SYBR FAST qPCR Kits for Bio-Rad iCycler^?contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition.

The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.

应用

KAPA2G Fast HotStart^?ReadyMix^?has been used for:

• Gene expression
• Gene knockdown validation
• Microarray validation
• ChIP analysis
• Low copy detection
• Absolute quantification of NGS libraries
• Real-time quantitative reverse transcription-PCR (qRT-PCR)
• quantitative PCR (qPCR)

特点和优势

Quantitate changes in gene expression more accurately

• High reaction efficiency between 95 - 105% improves accuracy and reproducibility.
• Unbiased efficiency across a wide range of GC contents and amplicon lengths.

Detect low copy and difficult targets consistently

• Improved processivity results in earlier Cq scores.
• Higher fluorescence detection across varying AT- and GC-rich targets.
• Novel enzyme is resistant to SYBR^?inhibition.

Complete real-time PCR runs in just 40 minutes

• 55% shorter run times with fast cycling protocol.
• Maintain high performance when switching from standard to fast protocols.

Quick Notes:

• This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
• The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
• 20 sec initial denaturation at 95℃ is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.
• For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72℃.
• Do not exceed 25 μL reaction volumes.

质量

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.

制备说明

Always ensure that components have been fully thawed and thoroughly mixed before use. KAPA SYBR^?FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20℃.

The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) for Bio-Rad^?iCycler^?and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20℃ when not in use. When protected from light, reagents are stable in the dark at 4℃ for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.

其他说明

For Research Use Only. Not for use in diagnostic procedures.

法律信息

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iCycler is a registered trademark of Bio-Rad

基本信息

NACRES

NA.55

半岛bd体育手机客户端 性质

质量水平	100
用途	sufficient for 100 reactions sufficient for 1000 reactions sufficient for 500 reactions
保质期	≤12 mo.
特点	dNTPs included hotstart: no
包装	kit of 1 mL (100 x 20 μL rxn; KK4606) kit of 10 mL (1000 x 20 μL rxn; KK4608) kit of 5 mL (500 x 20 μL rxn; KK4607)
manufacturer/tradename	Roche
浓度	2 ×
technique(s)	qPCR: suitable
input	purified DNA
相容性	for use with Bio-Rad iCycler
detection method	SYBR?Green
运输	dry ice
储存温度	?20℃

组份列表

组份不可单独销售

货号	组份
	KAPA SYBR?FAST DNA Polymerase
	reaction buffer
	dNTPs
	SYBR?Green I dye
	MgCl22.5 mM

安全信息

象形图
警示用语：	Warning
危险声明	H371
预防措施声明	P260 - P264 - P270 - P308 + P311 - P405 - P501
危险分类	STOT SE 2
储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	does not flash
闪点(C)	does not flash