半岛bd体育手机客户端 说明
一般描述
KAPA PROBE FAST qPCR预混液(2X) ABI Prism?试剂盒为所有基于探针的 qPCR 应用提供快速且可重复的结果。试剂盒包含即用型预混液,可使用序列特异性荧光探针化学,包括水解探针(例如,TaqMan?)、FRET 探针和置换探针(例如,分子信标),实现高灵敏度和高准确度的实时 PCR。
应用
KAPA PROBE FAST qPCR预混液 (2X) ABI PRISM?适用于:
特点和优势
准确区分等位基因:
质量
KAPA PROBE FAST qPCR Master Mix (2X) ABI PRISM?经过严格的质量控制检测,不含污染的外切和核酸内切酶活性,并且符合 DNA 污染水平方面的严格要求。
制备说明
ROX/荧光素引用染料(取决于试剂盒配置)对光敏感。长时间暴露于直射光下会导致荧光信号强度的损失。避免反复冻融。使用前务必确保半岛bd体育手机客户端 已完全解冻并混合。
KAPA PROBE FAST qPCR Master Mix (2X) ABI PRISM?通过30次冻融循环可稳定保存。确保所有试剂在不使用时均避光储存于-20℃ 条件下。避光条件下,试剂在 4℃ 黑暗环境中至少可稳定保存一周,只要确保不被微生物和/或核酸酶污染,短期内使用可在此温度下储存。
其他说明
仅供研究使用。不可用于诊断操作。
法律信息
ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Prism is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
基本信息
NACRES | NA.55 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
保质期 | ≤12 mo. |
包装 | kit of 1 mL (100 x 20 μL rxn; KK4705) kit of 10 mL (1000 x 20 μL rxn; KK4707) kit of 5 mL (500 x 20 μL rxn; KK4706) kit of 50 mL (5000 x 20 mL rxn; KK4714) pkg of |
manufacturer/tradename | Roche |
浓度 | 2 × |
technique(s) | qPCR: suitable |
相容性 | for use with Applied Biosystems?5700 for use with Applied Biosystems?7000 for use with Applied Biosystems?7300 for use with Applied Biosystems?7700 for use with Applied Biosystems?7900HT for use with StepOne? for use with StepOnePlus? |
运输 | wet ice |
储存温度 | ?20℃ |
组份列表
组份不可单独销售
货号 | 组份 |
qPCR Master Mix | |
ROX?High incorporated in the reaction mix | |
KAPA Taq HotStart?DNA Polymerase | |
dNTPs | |
MgCl25 nM |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | does not flash |
闪点(C) | does not flash |