Insulin-Transferrin-Sodium Selenite Supplement

半岛bd体育手机客户端 说明

一般描述

The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture.
The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio.
Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic

应用

The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added.

制备说明

Working concentration:Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10^-8M)

Storage conditions (working solution):-15 to -25 ℃.
Prepare appropriate aliquots and avoid repeated freezing and thawing.

重悬

The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution).
Further dilution with culture medium.

其他说明

For life science research only. Not for use in diagnostic procedures.

半岛bd体育手机客户端 性质

质量水平	100
生物来源	human serum (transferrin) synthetic (sodium selenite)
重组	expressed in human cells
无菌性	non-sterile; 0.2 μm filtered
测定	>98% (Transferrin, SDS-PAGE) >99% (Insulin, HPLC) >99% (Sodium selenite)
形式	lyophilized
包装	pkg of 50 mg (for 5 l medium)
manufacturer/tradename	Roche
technique(s)	cell culture | mammalian: suitable
溶解性	water: miscible
UniProt登记号	P01317 P02786
运输	wet ice
储存温度	2-8℃
Gene Information	bovine ... INS(280829) human ... TFRC(7037)

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	does not flash
闪点(C)	does not flash