半岛bd体育手机客户端 说明
一般描述
Recombinant, human granzyme A expressed inE. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed inE. coli.
Recombinant, human granzyme A expressed inE. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1β. Note: The M.W. is that of a homodimer.
包装
10 μg in Plastic ampoule
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.
外形
In 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.
其他说明
Fan, Z., et al. 2002.Mol. Cell. Biol.22,2810.
Russell, J.H., and Ley, T. J. 2002.Annu. Rev. Immunol.20,323.
Beresford, P.J., et al. 2001.J. Biol. Chem.276,43285.
Simon, M.M., and Kramer, M.D. 1994.Methods Enzymol.244,68.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 酶学委员会(EC)编号 | 3.4.21.78 ( BRENDA | IUBMB ) |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 重组 | expressed inE. coli |
| 形式 | liquid |
| specific activity | ≥100 units/μg protein |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 运输 | wet ice |
| 储存温度 | ?70℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
