GC-RICH PCR System, dNTPack

半岛bd体育手机客户端 说明

一般描述

The GC-RICH PCR System, dNTPack is composed of a special enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR reaction buffer in combination with the included GC-RICH resolution solution allows to amplify very efficiently difficult templates like GC-rich targets.

应用

The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates.
The GC-RICH PCR System, dNTPack may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA Polymerase alone.

特点和优势

• Ease access to difficult templates:-

including GC-rich targets and repetitive sequences.

• Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
• Amplify DNA fragments up to 5 kb.
• Cost-effective:-

use the convenient premixed solution of PCR grade NTPs.

包装

1 kit containing 6 components

质量

The GC-RICH PCR System is function-tested, by amplifying a human 284 bp ApoE genomic fragment, using the GC-RICH PCR System protocols.

单位定义

Volume Activity: 2 U/μl

制备说明

Working concentration:The optimal enzyme concentration range from 0.5 to 5 U per assay. For a standard 50 μl PCR, we recommend using 2 U of the enzyme blend.

其他说明

仅用于生命科学研究。不可用于诊断。

法律信息

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

半岛bd体育手机客户端 性质

用途	sufficient for ≤50 reactions
特点	Difficult Templates/Specialty Enzymes PCR dNTPs included hotstart: no
包装	pkg of 100 U
manufacturer/tradename	Roche
参数	72 ℃ optimum reaction temp.
technique(s)	PCR: suitable
input	purified DNA
运输	dry ice
储存温度	?20℃ (?15℃ to ?25℃)

组份列表

组份不可单独销售

货号	组份
	GC-RICH Enzyme Mix, in storage buffer
	GC-RICH PCR Reaction Buffer, with 7.5 mM MgCl2and DMSO 5x concentrated
	GC-RICH Resolution Solution 5 M
	MgCl2Stock Solution 25 mM
	Water, PCR Grade
	PCR Nucleotide Mix

安全信息

危险声明	H412
预防措施声明	P273 - P501
危险分类	Aquatic Chronic 3
储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	does not flash
闪点(C)	does not flash