半岛bd体育手机客户端 说明
一般描述
A cell-permeable thiooxodihydropyrimidinedione compound that inhibits both formin-mediated, profilin-independent actin nucleation (IC50~15 ?M using mDia1 or mDia2) and formin-mediated elongation of actin filaments in the presence of profilin (IC50~4 ?M using Cdc12 or mDia2), but not the Arp2/3-mediated or formin-independent actin assembly. SMIFH2 targets the FH2 (formin homology 2) domain of formins from a large variety of species, including murine mDia1/2,C. elegansCYK-1,S. pombeCdc12,S. pombeFus1, andS. cerevisiaeBni1, and decreases formin affinity for the actin filament barbed end. SMIFH2, at 25 ?M, is shown to selectively disrupt formin-dependent actin cables and contractile rings, but not Arp2/3-dependent, CK-666- (Cat. No. 182515) sensitive actin patches, in fission yeast. SMIFH2 is also demonstrated to affect F-actin cytoskeleton structures and cell migration (by a 2-fold decrease at 10 ?M) in NIH 3T3 fibroblast cultures.
A cell-permeable thiooxodihydropyrimidinedione compound that inhibits both formin-mediated, profilin-independent actin nucleation (IC50~15 ?M using mDia1 or mDia2) and formin-mediated elongation of actin filaments in the presence of profilin (IC50~4 ?M using Cdc12 or mDia2), but not the Arp2/3-mediated or formin-independent actin assembly. SMIFH2 targets the FH2 (formin homology 2) domain of formins from a large variety of species, including murine mDia1/2,C. elegansCYK-1,S. pombeCdc12,S. pombeFus1, andS. cerevisiaeBni1, and decreases formin affinity for the actin filament barbed end. SMIFH2, at 25 ?M, is shown to selectively disrupt formin-dependent actin cables and contractile rings, but not Arp2/3-dependent, CK-666- (Cat. No. 182515) sensitive actin patches, in fission yeast. SMIFH2 is also demonstrated to affect F-actin cytoskeleton structures and cell migration (by a 2-fold decrease at 10 ?M) in NIH 3T3 fibroblast cultures.
包装
10 mg in Glass bottle
Packaged under inert gas
警告
Toxicity: Standard Handling (A)
重悬
Use only fresh DMSO. Following reconstiution, aliquot an freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
其他说明
Rizvi, S.A., et al. 2009.Chem. Biol.16,1158.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C15H9BrN2O3S |
| 分子量 | 377.21 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥98% (sum of two geometrical isomers, HPLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze protect from light |
| 颜色 | darkdeep green |
| 溶解性 | DMSO: 50 mg/mL |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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