Endo-β-Galactosidase,Bacteroides fragilis,Recombinant,E. coli

半岛bd体育手机客户端 说明

一般描述

Recombinant, Bacteroides fragilis endo-β-galactosidase expressed inE. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles theEscherichia freundiienzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide. Optimum pH: 5.8.
Recombinant,Bacteroides fragilisendo-β-galactosidase expressed inE. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles theEscherichia freundiienzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.

包装

50 μL in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will liberate one ?mol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.

外形

Sterile-filtered in 20 mM Tris-HCl, pH 7.5.

重悬

There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.

其他说明

Murata, T., et al. 2003.Eur. J. Biochem.270,3709.
Scudder, P., et al. 1986.Eur. J. Biochem.157,365.
Scudder, P., et al. 1984.J. Biol. Chem.259,6586.
Scudder, P., et al. 1983.Biochem. J.213,485.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

酶学委员会(EC)编号

3.2.1.103 ( BRENDA | IUBMB )

半岛bd体育手机客户端 性质

质量水平	100
重组	expressed inE. coli
形式	liquid
specific activity	≥14 U/mL ≥140 units/mg protein
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
运输	ambient
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable