DNA Molecular Weight Marker XVII (500 bp ladder)

半岛bd体育手机客户端 说明

一般描述

Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range:0.5 to 5.0 kbp.

应用

DNA Molecular Weight Marker XVII (500 bp ladder) allows accurate sizing of DNA fragments generated by PCR, RT-PCR or restriction digest, following separation on agarose gels. The DNA Molecular Weight Marker XVII can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder), DNA Molecular Weight Marker XIV (100 bp ladder), and DNA Molecular Weight Marker XVI (250 bp ladder) for precise size determination.

序列

The mixture contains 10 blunt-ended, double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 500, 1000, 1500,2000, 2500, 3000, 3500,4000, 4500, and 5000. The 2000- and 4000-bp banding pattern are three times brighter. Electrophoretic separation of this molecular weight marker results in a regular pattern.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The fragments exhibit uniform spacing for easier reading, while bands 2000 and 4000 are 3 times brighter for easy orientation.

单位定义

50 μg = 1A₂₆₀unit

外形

Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).

制备说明

Storage conditions (working solution):2 to 8 ℃
Once thawed, store at 2 to 8 ℃.

其他说明

仅用于生命科学研究。不可用于诊断。

半岛bd体育手机客户端 性质

形式	solution
质量水平	100
包装	pkg of 50 μg (in 200 μl)
manufacturer/tradename	Roche
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	does not flash
闪点(C)	does not flash