DNA Base Excision Repair Pathway Inhibitor-CAS 6960-45-8-Calbiochem

半岛bd体育手机客户端 说明

一般描述

A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC₅₀= ~3 ?M). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 ?M. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H₂O₂in HeLa, HT1080, and MDA-MB-231 cells.
A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC₅₀~3 ?M). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 ?M. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H₂O₂in HeLa, HT1080, and MDA-MB-231 cells.

包装

25 mg in Plastic ampoule
Packaged under inert gas

生化/生理作用

Cell permeable: yes
Primary Target
APE 1
Product does not compete with ATP.
Reversible: no
Target IC₅₀: ~3 ?M against APE1 (human apurinic/apyrimidinic endonuclease

警告

Toxicity: Irritant (B)

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他说明

Madhusudan, S., et al. 2005.Nucl. Acids Res.33,4711.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C9H6N2O4
分子量	206.15
MDL编号	MFCD00044720

半岛bd体育手机客户端 性质

质量水平	100
测定	≥98% (HPLC)
形式	solid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze protect from light
颜色	tan
溶解性	DMSO: 5 mg/mL
运输	ambient
储存温度	2-8℃
InChI	1S/C9H6N2O4/c12-9(13)6-4-5-2-1-3-7(11(14)15)8(5)10-6/h1-4,10H,(H,12,13)/p-1
InChI key	BIUCOFQROHIAEO-UHFFFAOYSA-M

安全信息

象形图
警示用语：	Danger
危险声明	H319 - H334
预防措施声明	P261 - P264 - P280 - P284 - P304 + P340 + P312 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Resp. Sens. 1
储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable