Dkk-1, Rat, Recombinant

半岛bd体育手机客户端 说明

一般描述

Recombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His?Tag sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.
Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His?Tag sequence, and expressed in NS0-derived mouse myeloma cells.

包装

10 μg in Glass bottle

生化/生理作用

ND?? = 0.1-0.5 ?g/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 0.2 ?m filtered PBS, BSA.

重悬

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 ?g/ml with sterile PBS containing ≥1% human or bovine serum albumin.

其他说明

Li, J. et al. 2006.Bone36,754.
Morvan, F. et al. 2006.J. Bone Miner. Res.21,934.
Niehrs, C. 2006.Oncogene25,7469.
Verani, R. et al. 2006.J. Neurochem.101,242.
Cappuccio, I. et al. 2005.J. Neurosci.25,2647.
Kemp, C. et al. 2005.Dev. Dyn.233,1064.
Adamska, M. et al. 2004.Dev. Biol.272,134.
Bullock, C.M. et al. 2004.Mol. Pharmacol.65,582.
Mao, B. et al. 2002.Nature417,664.
Mao, B. et al. 2001.Nature411,321.
Krupnik, V.E., et al. 1999.Gene238,301.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

重组	expressed in mouse NSO cells
质量水平	100
测定	≥95% (SDS-PAGE)
形式	lyophilized
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
杂质	≤1.0 EU/μg protein Endotoxin
运输	ambient
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 2