Dkk-1, Mouse, Recombinant

半岛bd体育手机客户端 说明

一般描述

Recombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND₅₀= 0.02-0.1 ?g/ml.

包装

10 μg in Glass bottle

生化/生理作用

ND?? = 0.02-0.1 ?g/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-inducedalkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 0.2 ?m sterile-filtered PBS, BSA.

重悬

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 ?g/ml in PBS containing at least 0.1% human or bovine serum albumin.

其他说明

Pinzone, J.J., et al. 2009.Blood113,517.
Li, J., et al. 2006.Bone36,754.
Morvan, F., et al. 2006.J. Bone Miner. Res.21,934.
Niehrs, C., 2006.Oncogene25,7469.
Verani, R., et al. 2006.J. Neurochem.101,242.
Cappuccio, I., et al. 2005.J. Neurosci.25,2647.
Kemp, C., et al. 2005.Dev. Dyn.233,1064.
Adamska, M., et al. 2004.Dev. Biol.272,134.
Bullock, C.M., et al. 2004.Mol. Pharmacol.65,582.
Mao, B., et al. 2002.Nature417,664.
Mao, B., et al. 2001.Nature411,321.
Glinka, A., et al. 1998.Nature391,357.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
重组	expressed in CHO cells
测定	≥95% (SDS-PAGE)
形式	liquid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
杂质	<1.0 EU/μg protein Endotoxin
运输	ambient
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3