半岛bd体育手机客户端 说明
一般描述
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+
特异性
This antibody recognizes the N-terminus of Histone H3 monomethylated at Lys36.
Wide species cross reactivity is predicted based on sequence similarity.
免疫原
Epitope: Monomethylated Histone H3 Lys36
KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 monomethylated at Lys36.
应用
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1e5 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 ?g of Normal Rabbit IgG, or 3 ?L (1 ?g) Anti-Monomethyl-Histone H3 (Lys36) and the Magna ChIP
Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated. Please refer to the Magna ChIP
Western Blotting Analysis:
HeLa acid extract and various recombinant Histone proteins were probed with Anti-monomethyl-Histone H3 (Lys36) (0.05 ?g /mL).
Immunoprecipitation Analysis:
10 ?g from a representative lot immunoprecipitated monomethyl-Histone H3 (Lys36) from HeLa acid extract.
10 ?g of this antibody immunoprecipitated monomethyl -Histone H3 (Lys36) from 500 ?g of HeLa acid extract.
Arrow indicates monomethyl-Histone H3 (Lys36) (~17 kDa).
Dot Blot Specificity Analysis:
Unmodified and various modified Histone peptides were probed with Anti-monomethyl-Histone H3 (Lys36) (0.5 ?g/mL).Histone peptides with various modifications were transferred to PVDF membrane (Cat. #: 16-665) and probed with Anti-monomethyl-Histone H3 (Lys36) at (0.5 ?g/mL). Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Chromatin Biology
The ChIPAb+ Monomethyl-Histone H3 (Lys36) set includes the monomethyl-Histone H3 (Lys36) antibody, a Normal Rabbit IgG & control primers which amplify a 213 bp region of the human GAPDH coding region.
包装
25 assays per set. Recommended use: 3 μL (1 μg) of antibody per chromatin immunoprecipitation (dependent upon biological context).
质量
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1e5 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 ?g of either Normal Rabbit IgG, or 3 ?L (1 ?g) Anti-Monomethyl-Histone H3 (Lys36) and the Magna ChIP?HiSens Kit (Cat. # 17-10460).
Successful immunoprecipitation of monomethyl Histone H3 (Lys36) associated DNA fragments was verified by qPCR using ChIP Primers, GAPDH coding D2. Please refer to the MagnaChIP
目标描述
~17 kDa
外形
Anti-Monomethyl-Histone H3 (Lys36) (Rabbit Polyclonal). One vial containing 75 ?L 0.35 mg/mL purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4),150mM NaCl, and 0.05% sodium azide, before the addition of 30% glycerol. Store at -20°C.
Normal Rabbit IgG. One vial containing 125 ?g Rabbit IgG in 125 ?L storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers, GAPDH coding D2. One vial containing 75 μL of 5 μM of each primer specific for human GAPDH coding region. Store at -20°C.
FOR: GCC ATG TAG ACC CCT TGA AGA G
REV: ACT GGT TGA GCA CAG GGT ACT TTA T
储存及稳定性
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
Includes normal rabbit IgG and primers specific for human GAPDH coding region
法律信息
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.32 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified immunoglobulin |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human, rat, mouse |
manufacturer/tradename | ChIPAb+ Upstate? |
technique(s) | ChIP: suitable dot blot: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI登记号 | NP_003484 |
UniProt登记号 | Q16695 |
运输 | dry ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
Sigma-Aldrich