半岛bd体育手机客户端 说明
一般描述
HNF-3A, Hepatocyte nuclear factor 3-alpha (also called FOXA1) is member of the Forkhead family of winged-helix transcription factors. This protein family has around 50 known transcription factors and play an important role in the regulation of cell differentiation, organogenesis, and gene expression. High levels of expression of HNF-3A has been observed in tumors arising from both the prostate and breast. Here it is believed this protein interacts with the androgen receptor in prostate tumors and the estrogen receptor α in breast tumors. In the case of ERα, HNF-3A interacts with cis-regulatory regions in heterochromatin and enhances the interaction of ERα with chromatin.
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+
特异性
This antibody recognizes HNF-3A/FOXA1.
免疫原
Recombinant protein corresponding to the entire protein of human FOXA1
应用
Research Sub Category
Transcription Factors
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from Mouse liver tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 ?l of either normal rabbit IgG, or 2 ?l Anti-FOXA1 (Part No.CS207368) and the Magna ChIP
Please refer to the EZ-Magna ChIP
Western Blotting Analysis:
Representative lot data.
Human pancreas tissue lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-FOXA1 (0.5 ?g/mL).
Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and chemiluminescence detection system. (Figure 3)
Arrow indicates FOXA1 (~51 kDa).
Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of NIH/3T3 and HeLa cells using Anti-HNF-3A/FOXA1 (Red). Actin filaments have been labeled with Alexa Fluor
This ChIPAb+ FOXA1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Research Category
Epigenetics & Nuclear Function
包装
25 assays per set. Recommended use: 2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).
质量
Chromatin Immunoprecipitation:
Representative lot data
Sonicated chromatin prepared from Mouse liver tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 ?L of either normal rabbit IgG,or 2 ?L Anti-FOXA1 and the Magna ChIP?A Kit (Cat. # 17-610). Successful immunoprecipitation of FOXA1- associated DNA fragments was verified by qPCR using ChIP Primers, Mouse Hnf4α enhancer (Figure 1).
Please refer to the EZ-Magna ChIP
目标描述
~51 kDa
外形
Anti-FOXA1 (Rabbit Polyclonal). One vial containing 50 ?L of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide before the addition of glycerol to 30%. Store at -20°C.
Concentration: 0.35 mg/mL
Normal Rabbit IgG. One vial containing 125 ?g Rabbit IgG in 125 ?L storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers, Mouse Hnf4α enhancer. One vial containing 75 μL of 5 μM of each primer specific for Mouse Hnf4α enhancer. Store at -20°C.
FOR: TTC CAG CTG CCT TTA TCT CCC TGT
REV: TCT CCA CAC ATG TCC AGC AGC CT
Affinity purified
储存及稳定性
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
分析说明
Control
Includes normal rabbit IgG and primers specific for Mouse Hnf4α enhancer.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.32 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human, mouse |
manufacturer/tradename | ChIPAb+ Upstate? |
technique(s) | ChIP: suitable immunocytochemistry: suitable western blot: suitable |
NCBI登记号 | NP_004487 |
UniProt登记号 | P55317 |
运输 | dry ice |
Gene Information | human ... FOXA1(3169) |
安全信息
储存分类代码 | 10 - Combustible liquids |
Sigma-Aldrich