半岛bd体育手机客户端 说明
一般描述
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ CBX8 set includes a CBX8 antibody, a Normal Rabbit IgG, and positive control primers which amplify 138 bp of the human HOXA2 promoter region. The CBX8 antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of CBX8 associated chromatin.
The family of Heterochromatin Proteins ("Chromobox Homolog", CBX) are highly conserved adapter molecules, which have important functions in the cell nucleus. These functions include gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.
特异性
Wide species reactivity expected based upon conservation of immunogen sequence
免疫原
Epitope: Human CBX8 (a.a. 350--389)
应用
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 ?g of either Normal Rabbit IgG (Part No. PP64B), or 2 ?g Anti-CBX8 (Part No. CS207348) and the Magna ChIP
Please refer to the Magna ChIP
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min ( 1e5 and 1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 ?g of either Normal Rabbit IgG (Part No. PP64B), or 2 ?g Anti-CBX8 (Part No. CS207348) and the Magna ChIP
Please refer to the MagnaChIP
Western Blotting Analysis:0.1 ?g/mL of this antibody detected CBX8 on 5, 15 and 50 ?g of HeLa whole cell lysate and 50 ?g of 293T whole cell lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Use ChIPAb+ CBX8 ChIP Validated rabbit polyclonal antibody for the detection of CBX8 also known as Chromobox protein homolog 8 & Polycomb 3 homolog.
包装
25 assays per set. Recommended use: 2.0 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
质量
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 ?g of either Normal Rabbit IgG (Part No. PP64B), or 2 ?g Anti-CBX8 (Part No. CS207348) and the Magna ChIP?HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of CBX8 associated DNA fragments was verified by qPCR using ChIP Primers, HoxA2 upstream (Part No. CS203200).
Please refer to the MagnaChIP
目标描述
~43kDa
外形
Format: Purified
Affinity purified
Anti-CBX8 (Rabbit Polyclonal), Part No. CS207348. One vial containing 50 ?g affinity purified rabbit polyclonal in 72 uL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7 mg/ml final). Store at -20°C.
Normal Rabbit IgG, Part No. PP64B. One vial containing 125 μg of purified Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers, HOXA2 Upstream. Part No. CS203200. One vial containing 75 μL of 5 μM of each primer specific for human HOXA2 promoter region (chr7:27142948-27143085, hg19 build). Store at -20°C.
FOR: AGG AAA GAT TTT GGT TGG GAA G
REV: AAA AAG AGG GAA AGG GAC AGA C
储存及稳定性
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
分析说明
Control
Includes normal rabbit IgG and primers specific for the human HOXA2 promoter region.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.32 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 克隆 | polyclonal |
| species reactivity | human |
| manufacturer/tradename | ChIPAb+ Upstate? |
| technique(s) | ChIP: suitable western blot: suitable |
| NCBI登记号 | NP_065700 |
| UniProt登记号 | Q9HC52 |
| 运输 | dry ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
Sigma-Aldrich
m.cnreagent.com
