半岛bd体育手机客户端 说明
一般描述
Homozygous: No
Consanguineous: No
细胞系描述
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA图谱分析
HLA DRB1: 1001 11
HLA DRB3: 202
HLA DQAP: 1.3.4.1
HLA DQA1: 0501 0104
HLA DQB1: 0501 0301
HLA DPB1: 02012 3201
血清学分析
HLA A: 11 19
HLA B: 7 40
培养基
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
传代培养常规
Maintain cultures between 3x105- 2x106cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | human blood |
种族 | Thai |
生长模式 | Suspension |
核型 | Not specified |
形态学 | Lymphoblastoid |
运输 | dry ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich