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CBTF

品牌
Sigma-Aldrich
货号
807664
包装型号
规格纯度
价格
1764.16 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
CBTF
APN-TFS ester
Sodium 4-((4-(cyanoethynyl)benzoyl)oxy)-2,3,5,6-tetrafluorobenzenesulfonate
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

CBTF (Sodium 4-((4-(cyanoethynyl)benzoyl)oxy)-2,3,5,6-tetrafluorobenzenesulfonate) is a heterobifunctional reagent. It bears a 3-arylpropionitrile (APN) group. CBTF can be prepared from 4-iodobenzoic acid. It participates in various amine-to-thiol coupling reactions. It participates in the synthesis of various antibody-dye conjugates. CBTF is widely used for antibody conjugation.

应用

CBTF is a bifunctional crosslinker for amine-to-thiol coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. CBTF readily reacts with organic amines in basic conditions to produce APN-probes suitable for cysteine labeling.

包装

50 mg in glass bottle

其他说明

Standard protein labeling procedure (lysine labeling):

  • Dissolve the protein in an appropriate buffer* with pH 7.5-9.0 (e.g. PBS) at 1-10mg/mL concentration.
  • Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per lysine residue).
  • Incubate at room temperature for 2 hours.
  • If necessary, purify the protein-APN conjugate using size exclusion chromatography or ultrafiltration.
  • The conjugate can be readily coupled with thiol-containing substrates by incubating the components in aqueous buffer (pH 6.5-9.0) at ambient temperature for 2 hours.



Standard protein labeling procedure (cysteine labeling):

Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10mg/mL concentration.
Apply the appropriate amount of the stock solution of the APN-labeled molecule (1-5 molar eq. per free cysteine residue).
Incubate at room temperature for 2 hours.
If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

*Note: avoid amine- and thiol-containing buffers.

基本信息

经验(实验)分子式 C16H4F4NNaO5S
分子量 421.26
PubChem化学物质编号 329768745
NACRES NA.22

半岛bd体育手机客户端 性质

形式 powder
质量水平 100
reaction suitability reagent type: cross-linking reagent
储存温度 ?20℃
SMILES string O=C(OC1=C(F)C(F)=C(S(=O)(O[Na])=O)C(F)=C1F)C2=CC=C(C#CC#N)C=C2
InChI 1S/C16H5F4NO5S.Na/c17-10-12(19)15(27(23,24)25)13(20)11(18)14(10)26-16(22)9-5-3-8(4-6-9)2-1-7-21;/h3-6H,(H,23,24,25);/q;+1/p-1
InChI key YFJYSJRZDOWXDH-UHFFFAOYSA-M

安全信息

象形图 GHS06
警示用语: Danger
危险声明 H301
预防措施声明 P301 + P310 + P330
危险分类 Acute Tox. 3 Oral
储存分类代码 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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