Cathepsin V, His·Tag^?, Human, Recombinant, NSO Cells

半岛bd体育手机客户端 说明

一般描述

Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His?Tag sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.
Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His?Tag sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.

包装

10 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.

外形

In 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.

重悬

To activate rhCathepsin V, pre-incubate it at 20 ?g/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).

其他说明

Tolosa, E., et al. 2003.J. Clin. Invest.112,517.
Turk, V., et al. 2001.EMBO J.20,4629.
Somoza, J.R., et al. 2000.Biochemistry39,12543.
Bromme, D., et al. 1999.Biochemistry38,2377.
Adachi, W., et al. 1998.Invest. Ophthalmol. Vis. Sci.39,1789.
Santamaria, I., et al. 1998.Cancer Res.58,1624.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

酶学委员会(EC)编号

3.4.22.43 ( BRENDA | IUBMB )

半岛bd体育手机客户端 性质

质量水平	100
重组	expressed in NSO cells
测定	≥95% (SDS-PAGE)
形式	frozen liquid
specific activity	≥300 units/μg
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
杂质	≤1.0 EU/μg Endotoxins (EU/μg Cathepsin V)
运输	wet ice
储存温度	?70℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable