Cathepsin K, His·Tag^?, Human, Recombinant,E. coli

半岛bd体育手机客户端 说明

一般描述

Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts. Recently reported to also be expressed in fibroblasts. Irregularities in activity contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. Contains a C-terminal His?Tag .
Note: 1 mU = 1 milliunit.
Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts and recent studies also suggest its expression in fibroblasts. Irregularities in cathepsin K activity, either too high or too low, contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. This recombinant enzyme contains a C-terminal His?Tag sequence.

包装

25 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will hydrolyze 0.4 ?mol Z-FR-AMC per min at 37°C, pH 5.5.

外形

In 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Delaisse, J.M., et al. 2003.Microsc. Res. Tech.61,504.
Dodds, R.A. 2003.Cell Biochem. Funct.21,231.
Everts, V., et al. 2003.Calcif. Tissue Int.73,380.
Hou, W.S., et al. 2003.Biol. Chem.384,891.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

酶学委员会(EC)编号

3.4.22.38 ( BRENDA | IUBMB )

半岛bd体育手机客户端 性质

质量水平	100
重组	expressed inE. coli
测定	≥95% (SDS-PAGE)
形式	liquid
specific activity	≥900 mU/mg protein
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
运输	wet ice
储存温度	?70℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable