半岛bd体育手机客户端 说明
一般描述
Recombinant, human c-MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed inS. frugiperdainsect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). c-MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
Recombinant, human MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed inS. frugiperdainsect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.
包装
10 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala, Glu, Lys, Tyr)6:2:5:1per min at 30°C, using variable concentrations of ATP (0.1-1.48 ?M).
外形
In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Palka, H.L., et al. 2003.J. Biol. Chem.278,5278.
Vadnais, J., et al. 2002.J. Biol. Chem.277,48342.
Crostella, L., et al. 2001.Oncogene20,3735.
Guiton, M., et al. 1994.J. Biol. Chem269,30370.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
质量水平 | 100 |
重组 | expressed in baculovirus infectedSf21 cells |
形式 | liquid |
specific activity | ≥30 units/mg protein |
manufacturer/tradename | Calbiochem? |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
运输 | wet ice |
储存温度 | ?70℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
Sigma-Aldrich