半岛bd体育手机客户端 说明
一般描述
BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.
应用
BM-Condimed is used as a supplement (10%, v/v) to the normal culture basal medium (e.g., F 10, M 199, RPMI 1640, DMEM), containing 10 to 20% FCS for the cultivation of human amniotic and chorionic villi cells. BM-Condimed was also shown to improve culture conditions for bone marrow cells and peripheral blood leukocytes.
外形
Physiological solution, filtered through a 0.2 μm pore size membrane, buffered with sodium bicarbonate.
制备说明
Working concentration:1 to 100μg/ml
1 to 100μg/ml
Working solution: Note:To 90ml of the normal basal medium F 10, M 199 Hanks, RPMI 1640, MEM Dulbecco, MEM Earle, Alpha MEM (containing 10 to 20% fetal bovine serum), add 10ml BM-Condimed. Do not use as a basal medium or as a replacement for serum. One percent L-glutamine may be added to the mixture.
Biological activity:Each lot is assayed for its ability to stimulate the colony growth of primary amniotic cells.
Source:BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.
Note:Do not add antibiotics, as these are already present in BM-Condimed, and additional antibiotics will affect the growth of the cells.
储存及稳定性
Store at 2 to 8 ℃. (Store in dark!)
其他说明
仅用于生命科学研究。不可用于诊断。
半岛bd体育手机客户端 性质
无菌性 | 0.2 μm filtered |
质量水平 | 100 |
形式 | solution |
包装 | pkg of 100 mL |
manufacturer/tradename | Roche |
technique(s) | cell culture | mammalian: suitable |
溶解性 | water: miscible |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | No data available |
闪点(C) | No data available |