BIONi018-A

半岛bd体育手机客户端 说明

一般描述

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

细胞系来源

Depositor
Bioneer

细胞系描述

Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell
Primary cell developmental stage: Adult
Passage number reprogrammed: 3
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
LIN28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control.
Vector map: File

Characterization
Analysis of Undifferentiated Cells
Marker expression: GABRB3 (+)DMNT3B (+)POU5F1 (OCT-4) (+)GDF3 (+)NANOG (+)TDG1 (+) SSEA-4 (+)SSEA-4 (+)SSEA-1 (-)SOX2 (+)TRA 1-81 (+)
Differentiation potency
Ectoderm: In vitro directed differentiation
Marker Expressed:Nestin (+)Pax6 (+)
Endoderm: In vitro directed differentiation
Marker Expressed:CD184 (+)Sox17 (+)
Mesoderm: In vitro directed differentiation
Marker Expressed:CD34 (+)CD56 (+)

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: 11
Cell line karyotype: 46, XY, normal male karyotype
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

联系

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

传代培养常规

Medium: Essential E8^?
Passage method: EDTA
Matrix: Matrigel^?/ Geltrex^?
CO₂concentration: 5%
O₂concentration: 5%
Temperature: 37℃

法律信息

E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.

半岛bd体育手机客户端 性质

生物来源	human adipose-derived MSC
质量水平	100
重编程方法	episomal
描述	age (20-24)
manufacturer/tradename	EBiSC?
性别	male
生长模式	adherent (pluripotent)
technique(s)	cell culture | stem cell: suitable
相关疾病	none
运输	dry ice
储存温度	?196℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable