Arp2/3 Complex Inhibitor I, CK-666-CAS 442633-00-3-Calbiochem

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一般描述

A cell-permeable indolyl-fluorobenzamide compound that selectively inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human, bovine, fission yeastS. pombe, and budding yeastS. cerevisiaeorigin (IC₅₀= 4, 17, 5, and 12 ?M, respectively), without affectingS. pombeformin domain Cdc12(FH2)-mediated or the spontaneous actin polymerization. Shown to inhibit the actin filament "comet tails" formation around intracellularListeriain infected SKOV3 cells (IC₅₀= 7 ?M) in a reversible manner. Structrual studies indicate that CK-666 targets a pocket formed between subdomain 4 of Arp2 and subdomain 1 of Arp3, preventing Arp2/3 from shifting into an active conformation upon N-WASP-VCA binding. CK-666 and CK-869 (Cat. No. 182516) exhibit different modes of binding, resulting in their different yeast cross-reactivities. CK-689 (Cat. No. 182517) can serve as a negative control.
A cell-permeable indolyl-fluorobenzamide compound that selectively inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human, bovine, fission yeastS. pombe, and budding yeastS. cerevisiaeorigin (IC₅₀= 4, 17, 5, and 12 ?M, respectively), without affectingS. pombeformin domain Cdc12(FH2)-mediated or the spontaneous actin polymerization. Shown to inhibit the actin filament "comet tails" formation around intracellularListeriain infected SKOV3 cells (IC₅₀= 7 ?M) in a reversible manner. Structrual studies indicate that CK-666 targets a pocket formed between subdomain 4 of Arp2 and subdomain 1 of Arp3, preventing Arp2/3 from shifting into an active conformation upon N-WASP-VCA binding. CK-666 and CK-869 (Cat. No. 182516) exhibit different modes of binding, resulting in their different yeast cross-reactivities. CK-689 (Cat. No. 182517) can serve as a negative control.

包装

25 mg in Glass bottle
Packaged under inert gas

警告

Toxicity: Regulatory Review (Z)

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

其他说明

Nolen, B.J., et al. 2009.Nature460,1031.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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