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APN-Maleimide

品牌
Sigma-Aldrich
货号
806536
包装型号
规格纯度
价格
1055.87 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
APN-Maleimide
3-(4-(2,5-dioxo-2,5-dihydro-1 H-pyrrol-1-yl)phenyl)propiolonitrile
MAPN
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

应用

APN-Maleimide is a bifunctional crosslinker for thiol-to-thiol coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. Due to kinetic resolution the first thiol reacts exclusively with the maleimide residue producing the protein-APN conjugate. This conjugate can be readily coupled with thiol-containing molecules.

包装

50 mg in glass bottle

制备说明

Standard protein labeling procedure (cysteine labeling).

  • Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
  • Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
  • Incubate at room temperature for 10 minutes.
  • If necessary, purify the protein-APN conjugate using size exclusion chromatography or ultrafiltration.
  • The conjugate can be readily coupled with thiol-containing substrates by incubating the components in aqueous buffer (pH 6.5-9.0) at ambient temperature for 2 hours.
*Note: avoid thiol-containing buffers.

基本信息

经验(实验)分子式 C13H6N2O2
分子量 222.20
PubChem化学物质编号 329769017
NACRES NA.22

半岛bd体育手机客户端 性质

形式 powder
质量水平 100
储存温度 2-8℃
SMILES string N#CC#CC1=CC=C(N2C(C=CC2=O)=O)C=C1
InChI 1S/C13H6N2O2/c14-9-1-2-10-3-5-11(6-4-10)15-12(16)7-8-13(15)17/h3-8H
InChI key CHKKXKRQICWZFF-UHFFFAOYSA-N

安全信息

象形图 GHS07
警示用语: Warning
危险声明 H302 - H315 - H319 - H335
预防措施声明 P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
危险分类 Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官 Respiratory system
储存分类代码 11 - Combustible Solids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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