半岛bd体育手机客户端 说明
一般描述
Terminal uridylyltransferase 4 (EC 2.7.7.52; UniProt Q5TAX3; also known as TUTase 4, Zinc finger CCHC domain-containing protein 11) is encoded by the ZCCHC11 (also known as KIAA0191, TUT4) gene (Gene ID 23318) in human. TUTase 4 is an uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. It is involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets and also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, and miR-143 and miR-200c. It is reported to catalyze the 3′ uridylation of miR-26A that targets IL6 transcript to abrogate the silencing of IL6 transcript and promote cytokine expression. It may also participate in toll-receptor signaling by suppressing TRAF6-dependent activation of NF-kappaB.
特异性
Clone 9F6.1 targets an epitope in the N-terminal region of ZCCHC11 (TUT4).
免疫原
GST-tagged recombinant human ZCCHC11 (TUT4) N-terminal fragment.
应用
Research Category
Epigenetics & Nuclear Function
Anti-ZCCHC11 (TUT4), clone 9F6.1, Cat. No. MABE1526, is a highly specific mouse monoclonal antibody that targets ZCCHC11 (TUT4) and has been tested in Western Blotting.
Western Blotting Analysis: 4 ?g/mL from a representative lot detected ZCCHC11 (TUT4) in 10 ?g of C2C12 mouse myoblast and Jurkat human T-cell lysates.
质量
Evaluated by Western Blotting in PC3 cell lysate.
Western Blotting Analysis: 4 ?g/mL of this antibody detected ZCCHC11 (TUT4) in 10 ?g of PC3 human prostate cancer cell lysate.
目标描述
~185 kDa observed. 185.2/81.14 kDa (human isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
外形
Protein G purified.
Format: Purified
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 9F6.1, monoclonal |
| species reactivity | human, mouse |
| technique(s) | western blot: suitable |
| 同位素/亚型 | IgG1κ |
| NCBI登记号 | NP_056084 |
| UniProt登记号 | Q5TAX3 |
| 运输 | ambient |
| Gene Information | human ... ZCCHC11(23318) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
