Anti-ZBTB46 (ZNF340) Antibody, clone 5A8.1

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一般描述

Zinc finger and BTB domain-containing protein 46 (UniProt Q86UZ6; also known as BTB-ZF protein expressed in effector lymphocytes, BTB/POZ domain-containing protein 4, BZEL, Classical dendritic cell-restricted zinc finger transcription factor zDC, Zinc finger protein 340) is encoded by the ZBTB46 (also known as BTBD4, ZNF340) gene (Gene ID 140685) in human. ZBTB46/ZNF340 is a broad complex, tramtrack, bric-a-brac and zinc finger (BTB-ZF) protein expressed in classical dendritic cells (cDCs), but not other immune cell populations, including plasmacytoid DCs, monocytes, or macrophages. Genome-wide ChIP-seq analysis reveals that ZBTB46/ZNF340 associates with more than 1,000 gene promoters and acts as a transcriptional repressor against many of these target genes. Zbtb46-knockout profoundly alters the cDC subset composition in the spleen in favor of CD8+ DCs, as well as upregulates activation pathways in steady-state cDCs, including elevated creb1 and MHC II expression, and enhances cDC production of vascular endothelial growth factor, leading to increased vascularization of skin-draining lymph nodes. Human ZBTB46/ZNF340 is a 589-a.a. protein containing a BTB domain (a.a. 31-99) and two C2H2-type Zinc fingers (a.a. 418-436, 446-468).

特异性

Clone 5A8.1 targets an internal epitope within the N-terminal half of human ZBTB46 (ZNF340).

免疫原

GST-tagged recombinant internal fragment from the N-terminal half of human ZBTB46 (ZNF340).

应用

Anti-ZBTB46 (ZNF340), clone 5A8.1 Antibody, Cat. No. MABE1035, is a highly specific mouse monoclonal antibody, that targets ZBTB46 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ZBTB46 (ZNF340) in human cererbral cortex, kidney, and tonsil tissue sections.

质量

Evaluated by Western Blotting in human ovary tissue lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected ZBTB46 (ZNF340) in 10 ?g of human ovary tissue lysate.

目标描述

~70 kDa observed. Target band size appears larger than the calculated molecular weight of 64.08 kDa Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified.
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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