Anti-WAPL Antibody, clone 1C7-B1

半岛bd体育手机客户端 说明

一般描述

Wings apart-like protein homolog (also known as Friend of Epstein-Barr virus nuclear protein 2) is a nuclear protein encoded by the WAPL (WAPAL) gene in human. Wapl functions as a negative regulator of cohesin–chromosome association and facilitates the progression of mitosis both during interphase and prophase. In interphase, Wapl destabilizes cohesin–chromosome interaction and thereby reduces the extent of cohesin-mediated chromosome looping. In prophase, mitotic kinases phosphorylate cohesin and its regulators. Wapl then triggers the release of phosphorylated cohesin from sister chromatids, allowing sister chromosome decatenation by topoisomerase II (Topo II). (PMID 24156811).

免疫原

Recombinant protein corresponding to human WAPL.

应用

Immunoprecipitation Analysis: 1.0 mL tissue culture supernantant of this antibody immunoprecipitated WAPL in HeLa cell lysate (Professor Haruhiko Siomi, Keio University School of Medicine).
Research Sub Category
Nuclear Receptors
Research Category
Epigenetics & Nuclear Function
Anti-WAPL Antibody, clone 1C7-B1 is an antibody against WAPL for use in Western Blotting, Immunoprecipitation.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected WAPL in 10 ?g of HeLa cell lysate.

目标描述

~175 kDa observed

外形

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	1C7-B1, monoclonal
species reactivity	human
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_055860
UniProt登记号	Q7Z5K2
运输	wet ice
Gene Information	human ... WAPAL(23063)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable