Anti-VHL Antibody, clone 11E12

半岛bd体育手机客户端 说明

一般描述

von Hippel-Lindau protein (VHL) is most widely recognized as belonging to an E3 ubiquitin ligase complex that specifically targets hypoxia-inducible factors (HIFs) for the purpose of polyubiquitination and proteolytic degradation. Within this complex, VHL acts as a target recruitment subunit for HIFα and its subsequent ubiquitination. The complex also contains proteins such as B and C elongins, Rbx1 and Cul2. Inactivation of VHL has been linked to renal cell carcinoma development, therefore suggesting that VHL plays a role as a tumor suppressor. VHL expression has been observed in both fetal and adult kidney and brain tissues. Defects in VHL expression are causal to renal cell carcinoma type 1 (RCC1), erythrocytosis familial type 2 (ECYT2), von Hippel-Lindau disease (VHLD), and pheochromocytoma.

免疫原

Purified human VHL

应用

Immunoprecipitation: It has been shown that this VHL clone will immunoprecipitate VHL from HEK 293 cells (Shoenfeld, 1998).
Research Category
Apoptosis & Cancer

Signaling
This Anti-VHL Antibody, clone 11E12 is validated for use in WB, IP for the detection of VHL.
Research Sub Category
Tumor Markers

Ubiquitin & Ubiquitin Metabolism

质量

Evaluated by Western Blot in mouse fetal brain tissue lysate.

Western Blot Analysis: 1 ?g/mL of the antibody detected VHL in 10 ?g of mouse fetal brain tissue lysate.

目标描述

~ 24 kDa observed MW. VHL SUMOylation may result in high MW bands (Cai, 2010).

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse fetal brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	11E12, monoclonal
species reactivity	human, mouse
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_000542
UniProt登记号	P40337
运输	wet ice
Gene Information	human ... VHL(7428)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable