Anti-TXNIP Antibody, clone JY2

半岛bd体育手机客户端 说明

一般描述

Thioredoxin-interacting protein (UniProt Q9H3M7; also known as Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1) is encoded by the TXNIP (also known as VDUP1) gene (Gene ID 10628) in human. Thioredoxin interacting protein (TXNIP) is an alpha-arrestin protein that acts as an oxidative stress mediator that binds thioredoxin active cysteine residue and suppresses its antioxidant activity. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. TXNIP over-expression results in increased cellular ROS level and oxidative cell death, while TXNIP suppression enhances cellular resistance to oxidative injury. TXNIP is also shown to inhibit cellular glucose uptake by downregulating plasma membrane localization of glucose transporter 1 (GLUT1). TXNIP is required for the maturation of natural killer cells. Its expression is markedly down-regulated in tumor cells and its levels are correlated with clinical stage of cancer. Mice with mutations or knockout of TXNIP gene are much more susceptible to carcinogenesis than wild-type mice, indicating its role in cancer suppression. Ref.: Masutani, H., et al. (2012). J. Clin. Biochem. Nutr. 50, 23-34.

特异性

Clone JY2 detected TXNIP in adult mouse cardiomyocytes. No TXNIP target band was detected in cardiomyocytes from Txnip-null mice (Yoshioka, J., et al. (2007). Circ. Res. 101(12):1328-1338).

免疫原

Recombinant full-length human TXNIP protein.

应用

Western Blotting Analysis: A representative lot detected full-length human TXNIP, but not human TXNIP constructs lacking C-terminal end a.a. 302-391 sequence, exogenously expressed in HEK293 cells (Yoshioka, J., et al. (2007). Circ. Res. 101(12):1328-1338).

Western Blotting Analysis: A representative lot detected TXNIP in adult mouse cardiomyocytes. No TXNIP target band was detected in cardiomyocytes from Txnip-null mice (Yoshioka, J., et al. (2007). Circ. Res. 101(12):1328-1338).
Detect TXNIP/VDUP1 using this mouse monoclonal Anti-TXNIP Antibody, clone JY2, Cat. No. MABS1225, validated for use in Western Blotting.
Research Category
Signaling

质量

Evaluated by Western Blotting in HeLa cells.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected TXNIP in 10 ?g of HeLa cell lysate.

目标描述

~53 kDa observed. 43.66/37.32 kDa (human isoform 1/2) and 44.36/44.29 kDa (mouse isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified.
Format: Purified
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	JY2, monoclonal
species reactivity	mouse, human
technique(s)	western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_006463.3
UniProt登记号	Q9H3M7
运输	ambient

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable