Anti-TRRAP Antibody, clone 2TRR-1B3

半岛bd体育手机客户端 说明

一般描述

Transformation/transcription domain-associated protein (UniProt Q9Y4A5; also known as 350/400 kDa PCAF-associated factor, PAF350/400, STAF40, TR-AP, Tra1 homolog) is encoded by the TRRAP (also known as PAF400) gene (Gene ID 8295) in human. Transformation/transcription domain-associated protein (TRRAP) is a cofactor component shared by several histone acetyltransferase (HAT) complexes, including SAGA (Spt-Ada-Gcn5-acetyltransferase), PCAF (P300/CBP-associated factor), and Tip60. TRRAP is an essential component of the histone epigenetic modifying mechinery that plays an important role in regulating cell-cycle progression. Trrap gene-knockdown or protein downregulation causes cell and mouse lethality, impaired spindle checkpoint and differentiation.

特异性

The immunogen sequence is present in both spliced isoforms of human TRRAP reported by UniProt (Q9Y4A5).

免疫原

Epitope: N-terminal to nuclear localization signal in the TP53-interacting region.
Ovalbumin-conjugated linear peptide corresponding to a sequence from the TP53-interacting region of human TRRAP.

应用

Research Category
Epigenetics & Nuclear Function
Anti-TRRAP Antibody, clone 2TRR-1B3 is an antibody against TRRAP for use in Western Blotting, Immunoprecipitation.
Immunoprecipitation Analysis: 10 ?L from a representative lot immunoprecipitated TRRAP from 1 mL (4.5 mg/mL) of HeLa nuclear extract (Courtesy of Dr. Mustapha OULAD-ABDELGHANI, IGBMC, France).
Immunoprecipitation Analysis: A representative lot immunodepleted TRRAP using nonhomologous end-joining/NHEJ-competent protein extracts prepared from human AAH-1 lymphoblastoid cells (Robert, F., et al. (2006). Mol. Cell. Biol. 26(2):402-412).
Research Sub Category
Histone Modifying Proteins

质量

Evalulated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRRAP in 10 ?g of HeLa nuclear extract.

目标描述

~400 kDa observed. 437.6 kDa (isoform 1) and 434.4 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

外形

Unpurified
Mouse monoclonal IgG2aκ ascites without preservatives.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	2TRR-1B3, monoclonal
species reactivity	human
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_001231509
UniProt登记号	Q9Y4A5
运输	dry ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable