半岛bd体育手机客户端 说明
一般描述
Eomesodermin homolog (UniProt O54839; also known as T-box brain protein 2, T-brain-2, TBR-2) is encoded by the Eomes (also known as Tbr2, C77258) gene (Gene ID 13813) in murine species. Tbr2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Trb2 has been shown to play a role in the development of the neocortex and functions in the differentiation pathway of radial glia to post-mitotic projection neuron.
特异性
Expected to react with both spliced isoforms of murine Tbr2.
免疫原
KLH-conjugated linear peptide corresponding to mouse Tbr2.
Epitope: Transcription Activation Domain
应用
This Anti-Tbr2 antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Tbr2.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Tbr2 in mouse cerebral cortex and mouse cerebellum tissue.
Western Blot (SNAP ID) Analysis: 0.5 ?g/ml from a previous lot detected Tbr2 in 10 ?g of E13-14 mouse brain lysate.
Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected Tbr2 in mouse and human brain.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
质量
Evaluated by Western Blot in E13-14 mouse brain lysate.
Western Blot Analysis: 0.05 ?g/ml of this antibody detected Tbr2 in 10 ?g of E13-14 mouse brain lysate.
目标描述
~85/95 kDa observed. Target bands appear larger than the calculated molecular size of 74.8 kDa (isoform 1) and 72.7 kDa (isoform 2).
外形
Affinity purified
Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
E13-14 mouse brain lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | chicken |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human, mouse, rat |
technique(s) | immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgY |
NCBI登记号 | NP_034266 |
UniProt登记号 | O54839 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich