Anti-SorLA Antibody, clone 20C11

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一般描述

Sortilin-related receptor (UniProt Q92673; also known as Gp250, LDLR relative with 11 ligand-binding repeats, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LR11, SorLA, SorLA-1, Sorting protein-related receptor containing LDLR class A repeats) is encoded by the SORL1 (also known as C11orf32) gene (Gene ID 6653) in human. SorLA and Sortilin are related Vps10p-domain (Vsp10p-D) family multi-ligand-binding receptors that target LPL, growth factors and peptides, as well as soluble amyloid precursor proein (sAPP) for endocytosis. Studies show that SorLA protects sAPP against proteolytic processing in compartments localized in the cell body (soma) of hippocampal neurons, whereas sortilin facilitates sAPP degradation by lysosomes localized in neurites. Hippocampal neurons lacking sortilin display reduced sAPPα production, suggesting that sortilin promotes α-secretase activity in neuronal systems. Unlike LRP1 and LRP1b, two other known sAPP-binding receptors, SorLA and Sortilin bind sAPP in a KPI domain-independent manner and their scavenging function therefore is not limited to sAPP produced from KPI-containing APP isoforms. Human SorLA is a single transmembrane (a.a. 2138-2158) protein with a 55-amino acid cytoplasmic tail (a.a. 2159-2214) and a large luminal/extracellular (a.a. 1-2137) region that contains a Vps10p-domain (Vsp10p-D; a.a. 1-731) with a signal peptide (a.a. 1-28) and a propeptide (a.a. 29-81) sequence at the N-terminal end and five BNR (bacterial neuraminidase repeat or (F/Y)WTD/b-propeller domain) repeats (a.a. 136-573). The C-terminal half of the luminal/extracellular region contains five LDL-R class B (LB) repeats (a.a. 800-1013), an EGF-like domain (a.a. 1026-1072), eleven LDL-R class A (LA) repeats (a.a. 1076-1551), and six fibronectin type-III repeats (a.a. 1557-2118).

特异性

Clone 20C11 immunostained SorLA endocytic vesicles in wild-type, but not Sorl1-/-, mouse astrocytes.

免疫原

Recombinant human SorLA extracellular/luminal domain.
Epitope: Extracellular/luminal region outside the Vps10p domain (Vsp10p-D).

应用

Anti-SorLA Antibody, clone 20C11 is an antibody against SorLA for use in Immunocytochemistry, Western Blotting, ELISA.
Immunocytochemistry Analysis: 5 ?g/mL from a representative lot immunostained SorLA endocytic vesicles in wild-type, but not Sorl1-/-, mouse astrocytes (Courtesy of Dr. Jakob V. Larsen, MIND-centre, University of Aarhus, Denmark).
Immunocytochemistry Analysis: A representative lot detected SorLA immunoreactivity primarily located at the cell body (soma) by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized primary murine hippocampal neurons (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).
Immunocytochemistry Analysis: A representative lot immunostained endocytic vesicular structures containing endocytosed sAPP by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells expressing exogenously transfected human SorLA (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).
Immunocytochemistry Analysis: A representative lot detected the cellular localization of exogenously expressed wild-type and the FANSHY-to-6A4 mutant human SorLA by fluorescent immuncytochemistry staining of 4% paraformaldehyde-fixed, 0.5% saponin-permeabilized SH-SY5Y transfectants (Fjorback, A.W., et al. (2012). J. Neurosci. 32(4):1467-1480).
Western Blotting Analysis: A representative lot detected endogenous SorLA in sucrose gradient-fractionated mouse brain extracts as well as the expression of exogenously transfected human SorLA in HEK293 transfectants (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).
ELISA Analysis: Representative lots were employed as the detection antibody for quantiating SorLA level in human brain cortical extracts as well as the level of exogenously expressed human SorLA in CHO cells (Caglayan, S., et al. (2012). Arch. Neurol. 69(3):373-379; Schmidt, V., et al. (2012). EMBO J. 31(1):187-200).

质量

Evaluated by Immunocytochemistry in human SorLA-expressing HEK293 cells.

Immunocytochemistry Analysis: 4.0 ?g/mL of this antibody immunostained SorLA endocytic vesicles in HEK293 cells expressing transfected human SorLA.

目标描述

239.3 kDa (human) and 238.3 kDa (mouse) calculated.

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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