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位置: 首页> 品牌> Sigma-Aldrich> Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II

Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II

品牌
Sigma-Aldrich
货号
AB5206
规格纯度
Chemicon?, from rabbit
价格
5688.25 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
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半岛bd体育手机客户端 名称:
Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II
Nav1.2, SCN2A
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

特异性

Recognizes type II alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested so far.

免疫原

Purified peptide of alpha subunit (amino acids 467-485) of rat type II voltage-gated sodium channel (VGSC) (Accession P04775).

应用

All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

Western blot: 1:200 using ECL on rat brain membranes. Note: The addition of 2% Triton to the antibody is recommended for this lot number.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II detects level of Sodium Channel & has been published & validated for use in IH & WB.

目标描述

228 kDa

外形

ImmunoAffinity Purified
Affinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

储存及稳定性

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

分析说明

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity purified immunoglobulin
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity mouse, rat
manufacturer/tradename Chemicon?
technique(s) immunohistochemistry: suitable
western blot: suitable
NCBI登记号 NM_001040142.1
NM_001040143.1
NM_021007.2
UniProt登记号 Q99250
运输 dry ice
Gene Information human ... SCN2A(6326)

安全信息

危险声明 H412
预防措施声明 P273 - P501
危险分类 Aquatic Chronic 3
储存分类代码 11 - Combustible Solids
WGK WGK 3

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