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Anti-SET-beta, clone 3D10E2

品牌
Sigma-Aldrich
货号
MABN1857
规格纯度
clone 3D10E2, from mouse
价格
5352.52 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-SET-beta, clone 3D10E2
Protein SET-beta, HLA-DR-associated protein II beta, IGAAD, beta isoform, Inhibitor of granzyme A-activated Dnase, beta isoform, PHAPII, beta isoform, I-2PP2A, beta isoform, Phosphatase 2A inhibitor I2PP2A, beta isoform, TAF-I beta, Template-activating f
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Protein SET (UniProt Q01105; also known as HLA-DR-associated protein II, IGAAD, Inhibitor of granzyme A-activated DNase, PHAPII, I-2PP2A, Phosphatase 2A inhibitor I2PP2A, TAF-I, Template-activating factor I) is encoded by the SET gene (Gene ID 6418) in human. Protein SET (patient “SE translocation”) adopted its name when it was originally identified in a leukemia study in 1992. SET interacts with signaling pathways that promote normal cell growth and proliferation, as well as tumorigenesis and metastasis. SET protein inhibits PP2A by enhancing lyase activity of P450c17, a substrate for PP2A when it is Ser- and Thr-phosphorylated. Alternative splicings result in multiple SET isoforms, including SET-alpha (TAF-I alpha), SET-beta (TAF-I beta), and a shorter spliced Set-β isoform without the nuclear localization signal (NLS). Set-β functions have been described both in the nucleus and cytoplasm. The presence of a nuclear localization signal (NLS) and phosphorylation govern Set-β isoforms cellular localization. Proteolytic cleavage of Set-β at Lys176 by granzyme A or at Asn-175 by asparagine endopeptidase results in the release of GzmA-activated DNase (GAAD) from a multiprotein complex. Uninhibited in the absence of the full-length Set-β, GAAD causes cell death by its DNA-nicking activity.

特异性

Clone 3D10E2 recognizes an epitope sequence specific to the spliced isoform SET-beta (TAF-I beta) of human, murine, and rat species (UniProt Q01105-2, Q9EQU5-2, Q63945-2). The epitope sequence is not present in SET-alpha (TAF-I alpha) or any other human, murine, and rat protein SET spliced isoforms reported by UniProt.

免疫原

KLH-conjugated linear peptide corresponding to a sequence near the N-terminus of human SET-beta.
Epitope: Near N-terminus.

应用

Western Blotting Analysis: 0.5 ?g/mL from a representative lot detected SET-beta in 10 ?g of LNCaP human prostate cancer cell lysate.
Western Blotting Analysis: 1.3 ?g/mL from a representative lot specifically detected SET-beta, but not SET-alpha in Ramos human Burkitt′s lymphoma cell lysate (Courtesy of M. P. Vitek, Ph.D. Dr. Vitek is an employee and stockholder in Oncotide Pharmaceuticals, Inc. and a faculty member of Duke University Medical Center. Matters of potential personal and institutional conflict of interest are managed by the Duke University Medical Center Conflict of Interest Committee).
Research Sub Category
Signaling Neuroscience
Research Category
Neuroscience
Anti-SET-beta, clone 3D10E2 is an antibody against SET-beta, clone 3D10E2 for use in Western Blotting.

质量

Evaluated by Western Blotting in DU145 cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected SET-beta in 10 ?g of DU145 human prostate cancer cell lysate.

目标描述

~36 kDa observed. Band size appears larger than the calculated molecular weight of 31.97 kDa (Met1 removed) due to posttranslational modification (acetylation and/or phosphorylation).

外形

Protein G purified.
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 3D10E2, monoclonal
species reactivity human, rat
species reactivity (predicted by homology) mouse (based on 100% sequence homology)
technique(s) western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_003002.2
UniProt登记号 Q01105-2
Gene Information human ... SET(6418)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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