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位置: 首页> 品牌> Sigma-Aldrich> Anti-Serpina3n Antibody, clone 13H5

Anti-Serpina3n Antibody, clone 13H5

品牌
Sigma-Aldrich
货号
MABC1182
规格纯度
clone 13H5, from hamster(Armenian)
价格
4634.66 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Serpina3n Antibody, clone 13H5
Serine protease inhibitor A3N
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Serine protease inhibitor A3N (UniProt: Q91WP6; also known as Serpin A3N) is encoded by the Serpina3n (also known as Spi2) gene (Gene ID: 20716) in murine species. SerpinA3N is a secreted protein of the serpin family. Serpins bind and inhibit specific serine proteases and several serine proteases are reported to be substrates for SerpinA3N in vitro. It is synthesized with a signal peptide of 20 amino acids that is cleaved to generate the mature protein. SerpinA3N is expressed at high levels in brain, heart, liver, lung, spleen, testis, and thymus. However, its expression is low in kidney, skeletal muscle, and bone marrow. Serpin A3N expression can also be induced by bacterial lipopolysaccharides. The reactive center loop (RCL) of as SerpinA3N extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the serpin reactive site and the protease. The resulting inactive serpin-protease complex is highly stable. SerpinA3N is shown to be up-regulated in the dorsal root ganglia after nerve injury and mice lacking SerpinA3N are reported to develop more neuropathic mechanical allodynia than wild-type (WT) mice. SerpinA3N is also shown to attenuate activated T cell-mediated neuronal death and reduce the severity of disease in experimental autoimmune encephalomyelitis (EAE). (Ref.: Vicuna, L et al. (2015) Nat. Med. 21(5):518-23).

特异性

Clone 13H5 specifically targets murine Serpina3n. It is predicted to react with both pro- and mature forms of Serpina3n. This clone targets a sequence within 24 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of murine Serpina3n.

应用

Immunofluorescence Analysis: A representative lot detected increased Serpin A3N/Serpina3n immunoreactivity in late metastasis stage lung tissue by fluorescent immunohistochemistry staining of paraformaldehyde-fixed frozen lung sections in a study employing the murine MMTV-PyMT breast-to-lung metastasis model (Sevenich, L., et al. (2014). Nat. Cell Biol. 16(9):876-888).

Research Category
Apoptosis & Cancer
Detect Serpina3n using this armenian hamster monoclonal Anti-Serpina3n Antibody, clone 13H5, Cat. No. MABC1182. It has been tested for use in Immunofluorescence and Western Blotting.

质量

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected Serpina3n in 10 ?g of mouse brain tissue lysate.

目标描述

~46 kDa observed; 46.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified armenian hamster monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

生物来源 hamster (Armenian)
质量水平 100
抗体形式 purified antibody
antibody product type primary antibodies
克隆 13H5, monoclonal
species reactivity mouse
should not react with human
technique(s) immunofluorescence: suitable
western blot: suitable
NCBI登记号 NP_033278.2
UniProt登记号 Q91WP6
运输 ambient

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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