Anti-RIP3 Antibody, clone 6E6.2

半岛bd体育手机客户端 说明

一般描述

Receptor-interacting protein 3 (RIP3) contains a N-terminal kinase domain and interacts with RIP. The binding of RIP to RIP3 causes the RIP3/RIP complex to be shuttled into the tumor necrosis factor receptor-1 signaling cascade. RIP3 is known to be a very effective apoptosis inducer. Two novel splice variants of RIP3 have been discovered, RIP3 beta and RIP3 gamma, that have shown to decrease apoptosis related to full length RIP3. RIP3 gamma has been shown to be significantly up-regulated in colon and lung cancers and may play a role in tumorigenesis.

特异性

This antibody recognizes RIP3 at the N-terminus.

免疫原

GST-tagged recombinant protein corresponding to human RIP3 at the N-terminus.
Epitope: N-terminus

应用

Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
This Anti-RIP3 Antibody, clone 6E6.2 is validated for use in WB, IH for the detection of RIP3.
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected RIP3 in 10 ?g of human placenta tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected RIP3 in rat pancreas tissue.

质量

Evaluated by Western Blot in human pancreas tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected RIP3 in 10 ?g of human pancreas tissue lysate.

目标描述

~56 kDa (isoform 1) and ~27 kDa (isoform 2) observed.
Isoform 3 (~25 kDa) may be observed in some lysates. An uncharacterized band may be observed at ~16 kDa in some lysates.

外形

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Human pancreas tissue lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	6E6.2, monoclonal
species reactivity	human, rat
technique(s)	immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_006862
UniProt登记号	Q9Y572
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable