Anti-Ptx3/PITX3 Antibody

半岛bd体育手机客户端 说明

一般描述

Pitx3, the third member of the Pitx family, is expressed in the eyes and in only one population of neurons, the dopaminergic neurons of the midbrain. In human, mutations of the Pitx3 gene have been associated with a familial form of cataracts, which is consistent with one of the developmental roles of this gene. Pitx3 expression in the midbrain starts just before terminal differentiation of dopaminergic neurons. This temporal expression is consistent with a role of Pitx3 in the differentiation and/or maintenance of these neurons.

特异性

Recognizes Ptx3 / PITX3 (Bicoid, Bcd family), a homeodomain transcription factor.

免疫原

Synthetic peptide

应用

Anti-Ptx3/PITX3 Antibody detects level of Ptx3/PITX3 & has been published & validated for use in WB, IH.
Research Category
Neuroscience
Western blot: 1:200-1:1,000 using ECL.

Immunohistochemistry: 1:200-1:1,000.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers

联系

Replaces: MABN1052

外形

Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

储存及稳定性

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	saturated ammonium sulfate (SAS) precipitated
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	rat, human, mouse
manufacturer/tradename	Chemicon?
technique(s)	immunohistochemistry: suitable western blot: suitable
NCBI登记号	NM_002852.2
UniProt登记号	P26022
运输	dry ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable