Anti-Prodynorphin Antibody

半岛bd体育手机客户端 说明

特异性

ProDynorphin, rat.

免疫原

Synthetic peptide from rat ProDynorphin.

应用

Anti-Prodynorphin Antibody is an antibody against Prodynorphin for use in IH.
Immunohistochemistry: 1:500

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5519

IMMUNOHISTOCHEMISTRY

Perfusion: 1) Ca2+-free Tyrode′s solution followed by 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5519. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

外形

Affinity purified Guinea pig antibody in PBS containing 0.05% sodium azide.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	guinea pig
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	rat
manufacturer/tradename	Chemicon?
technique(s)	immunohistochemistry: suitable
NCBI登记号	NM_024411.2
UniProt登记号	P01213
运输	dry ice
Gene Information	human ... PDYN(5173)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable