Anti-Pro-Atrial Natriuretic Peptide Antibody, clone 11E3.9

半岛bd体育手机客户端 说明

一般描述

Natriuretic peptides A (UniProt P01160; also known as Atriopeptin, Cardiodilatin, Cardiodilatin-related peptide, Cardionatrin, CDD, CDD-ANF, CDP, Natriuretic peptide precursor A, Prepronatriodilatin) is encoded by the NPPA (also known as ANF, ANP, ATFB6, ATRST2, PND) gene (Gene ID 4878) in human. Atrial natriuretic peptide (ANP), also called atrial natriuretic factor, is a cardiac hormone that reduces high blood pressure by promoting salt excretion, decreasing blood volume, and relaxing vessel tension in a receptor-dependent manner. Deficiency in either ANP or its receptor causes spontaneous hypertension in knockout mice. ANP is initially synthesized in cardiac myocytes as a 153 a.a. precursor with a signal (a.a.1-25) and a propeptide (a.a. 26-123) sequence. The removal of the signal peptide yields pro-ANP (a.a. 26-151), which is further cleaved by the transmembrane cardiac serine protease Corin to yield mature ANP (a.a. 124-151), also known as atrial natriuretic factor (ANF). ANP is a short-lived molecule partly due to its proteolytic degradation by the kidney Neprilysin, also known as neutral endopeptidase 24.11.

特异性

Expected to react with both prepro- and pro-atrial natriuretic peptide (ANP), but not mature ANP (a.a. 124-151 of prepro sequence).

免疫原

Epitope: Propeptide (a.a. 26-123)
His-tagged recombinant protein corresponding to human Pro-Atrial Natriuretic Peptide.

应用

Research Sub Category
Apoptosis - Additional
This Anti-Pro-Atrial Natriuretic Peptide Antibody, clone 11E3.9 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Pro-Atrial Natriuretic Peptide.
Western Blotting Analysis: 1.0 ?g/mL from a representative lot detected human Pro-Atrial Natriuretic Peptide in 10 ?g of human heart tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected human Pro-Atrial Natriuretic Peptide in rat cardiac myocytes.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in rat heart tissue lysate.

Western Blotting Analysis: 1.0 ?g/mL of this antibody detected human Pro-Atrial Natriuretic Peptide protein in 10 ?g of rat heart tissue lysate.

目标描述

~17 kDa observed

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	11E3.9, monoclonal
species reactivity	human, rat
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_006163
UniProt登记号	P01160
运输	wet ice
Gene Information	human ... NPPA(4878)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable