您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-PRMT9 Antibody, clone 128-29-1

Anti-PRMT9 Antibody, clone 128-29-1

品牌
Sigma-Aldrich
货号
MABE1112
规格纯度
clone 128-29-1, from mouse
价格
5352.52 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-PRMT9 Antibody, clone 128-29-1
Putative protein arginine N-methyltransferase 9, Putative protein arginine N-methyltransferase 10
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Putative protein arginine N-methyltransferase 9 (UniProt Q6P2P2; also known as Putative protein arginine N-methyltransferase 10) is encoded by the PRMT9 (also known as PRMT10) gene (Gene ID 90826) in human. Mammalian protein arginine methyltransferases (PRMTs) catalyze the methylation of arginine residues on histone and non-histone proteins. PRMTs are divided into three types based on their mode of activity. Type I PRMTs (PRMT1–4, PRMT6, and PRMT8) transfer up to two methyl groups on the same terminal guanidino nitrogen of arginine to form ω-NG-monomethylarginine (MMA) and ω-NG,NG-asymmetric dimethylarginine (ADMA) residues. Type II PRMTs (PRMT5 and PRMT9) transfer methyl groups on different terminal guanidino nitrogens to form ΜΜΑ and ω-NG,N′G-symmetric dimethylarginine (SDMA) residues, while type III PRMT7 transfers only one single methyl group to form MMA residues. PRMT9 is involved in alternative splicing regulation by modulating spliceosomal small nuclear ribonucleoprotein (snRNP) maturation in the cytoplasm. PRMT9 complexes with the splicing factors SF3B2 (SAP145) and SF3B4 (SAP49) and mediates SF3B2 Arg508 modification via MMA and SDMA. Similar to PRMT7, but unlike other PRMTs, PRMT9 contains a duplicated methyltransferase domain (a.a. 137-446 and 530-845), with the N-terminal MTase acting as a funtional AdoMet-binding domain and the C-terminal MTase domain serving a pseudodimer function, mimicking the homodimer formation of other PRMTs needed for subtrate processing. In addition, PRMT9 contains three tetratricopeptide repeats (TPR1-3; a.a. 25-58, 67-100, 101-134) known to mediate protein-protein interactions.

特异性

Clone detected GST fusion containing recombinant human PRMT9 fragment a.a. 401-550, as well as endogenous human, but not mouse, PRMT9 in cell lysates (Yang, Y., et al. (2015). Nat. Commun. 6:6428). Epitope region is present in both spliced isoforms of human PRMT9 reported by UniProt (Q6P2P2).

免疫原

GST-tagged recombinant protein corresponding to the N-terminal half of human PRMT9.
Epitope: First MTase domain.

应用

Research Sub Category
General Post-translation Modification
Anti-PRMT9 Antibody, clone 128-29-1 is an antibody against PRMT9 for use in Western Blotting, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry.
Western Blotting Analysis: 2.0 ?g/mL from a representative lot detected PRMT9 in 20 ?g of HeLa cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PRMT9 in human kidney, testis, and cerebellum tissue sections.
Immunocytochemistry Analysis: A representative lot detected a predominant cyotoplasmic PRMT9 localization by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed HeLa cells (Yang, Y., et al. (2015). Nat. Commun. 6:6428).
Immunoprecipitation Analysis: A representative lot immunoprecipitated PRMT9 from HeLa cell lysates (Yang, Y., et al. (2015). Nat. Commun. 6:6428).
Western Blotting Analysis: A representative lot detected GST fusion protein containing human PRMT9 a.a. 401-550 recombinant fragment, but not PRMT9 a.a. 1-199, 200-400, or 551-895 GST fusion (Yang, Y., et al. (2015). Nat. Commun. 6:6428).
Western Blotting Analysis: A representative lot detected human, but not mouse, PRMT9 using lysates from various human and mouse cell lines (Yang, Y., et al. (2015). Nat. Commun. 6:6428).
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected PRMT9 in 20 ?g of A431 cell lysate.

目标描述

~94 kDa observed. 94.50 kDa (isoform 1) and 81.86 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 128-29-1, monoclonal
species reactivity human
should not react with mouse
technique(s) immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_612373
UniProt登记号 Q6P2P2

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map