半岛bd体育手机客户端 说明
特异性
Polycystin-2.
免疫原
Synthetic peptide from the C-terminal of mouse Polycystin-2.
应用
Research Sub Category
Renal Physiology
Research Category
Metabolism
Western blot: 1:500 using ECL. The antibody reacts with the 105 kDa Polycystin-2 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.
Anti-Polycystin-2 Antibody is an antibody against Polycystin-2 for use in WB, IC, IH.
外形
Rabbit serum. Liquid.
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
分析说明
Control
POSITIVE CONTROL: adult mouse kidney
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | mouse |
manufacturer/tradename | Chemicon? |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
NCBI登记号 | NM_000297.2 |
UniProt登记号 | Q13563 |
运输 | dry ice |
Gene Information | human ... PKD2(353503) mouse ... Pkd2(18764) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich