Anti-Polycystin-1 Antibody, clone E4 (5F4D2)

半岛bd体育手机客户端 说明

一般描述

Polycystin-1 (UniProt P98161; also known as Autosomal dominant polycystic kidney disease 1 protein) is encoded by the PKD1 gene (Gene ID 5310) in human. The primary cilium is a microtubule-based mechano- and chemo-sensory organelle that coordinates an array of cellular pathways during development and in tissue homeostasis, including Hedgehog (Hh), PDGFRα and Wnt signaling. Defective ciliary function causes a variety of ciliopathies, including autosomal dominant polycystic kidney disease (ADPKD). Polycystin-1 (PC1) and polycystin-2 (PC2) are large transmembrane proteins co-localized to primary cilium, where they play an important role in calcium-based signalling. PC1 is an atypical adhesion G-protein-coupled receptor (aGPCR) with 11-transmemrbane domains. PC1 is cis-autoproteolytically cleaved at a juxtamembrane GPCR cleavage site (GPS; a.a. 3012-3061) in a developmental stage-dependent manner, where PC1 is largely uncleaved in early embryonic kidneys but becomes extensively cleaved after birth. GPS cleavage results in a heterodimeric PC1 form, in which the N-terminal fragment (NTF) remains non-covalently associated with the transmembrane C-terminal fragment (CTF). Transgenic mice expressing non-cleavable PC1 develop cystic kidney disease during the postnatal period.

特异性

Clone E4 (also known as clone 5F4D2) targets polycystin-1 extracellular C-type lectin domain and immunostained wild-type, but not Pkd1-knockout murine cells (Kim, H., et al. (2014). Nat. Commun. 5:5482).

免疫原

FLAG-tagged recombinant human polycystin-1 C-type lectin domain.
Epitope: C-type lectin domain.

应用

Anti-Polycystin-1 Antibody, clone E4 (5F4D2) is an antibody against Polycystin-1 for use in Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting, Immunocytochemistry.
Research Category
Signaling
Immunohistochemistry Analysis: An 1:50 dilution of this antibody from a representative lot detected polycystin-1 immunoreactivity in rat and mouse kidney tissue sections.
Immunoprecipitation Analysis: A representative lot immunoprecipitated polycystin-1 from mouse lung tissue lysates (Courtesy of Dr. Feng Qian, University of Maryland School of Medicine, Baltimore, MD).
Western Blotting Analysis: A representative lot detected exogenously expressed polycystin-1 in lysates from HEK cells transfected with human or mouse Pkd1 cDNA, but not in lysates from mock-transfected HEK cells (Courtesy of Dr. Feng Qian, University of Maryland School of Medicine, Baltimore, MD).
Western Blotting Analysis: A representative lot detected the polycystin-1 (PC1) GPS domain cis-autocleaved N-terminal fragment (NTF) in lysates from HEK cells transfected with full-length Pkd1 cDNA, but not in lysates from mock-transfected HEK cells (Kim, H., et al. (2014). Nat. Commun. 5:5482).
Immunocytochemistry Analysis: A representative lot and an anti-Arl13b antibody co-stained the primary cilium of 4% formaldehyde-fixed, 0.1% Triton X-100-permeabilized murine embryonic fibrolasts (mMEF) and collecting duct (CD)-derived cells by dual fluorescence immunocytochemistry. No polycystin-1 immunoreactivity was detected in Pkd1-knockout mMEFs (Kim, H., et al. (2014). Nat. Commun. 5:5482).

Research Sub Category
Developmental Signaling

质量

Evaluated by Immunohistochemistry in human kidney tissue.

Immunohistochemistry Analysis: An 1:250 dilution of this antibody lot detected polycystin-1 immunoreactivity in human kidney tissue.

目标描述

Calculated molecular weights of full-length/GPS domain autocleaved N-terminal fragment: 460.3/325.0 kDa (isoform 1), 459.1/324.0 kDa (isoform 2), 460.2/325.0 kDa (isoform 3).

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	E4 (5F4D2), monoclonal
species reactivity	rat, mouse, human
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_001009944.2
UniProt登记号	P98161
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable