您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-phospho-MLKL (Ser345) Antibody, clone 7C6.1

Anti-phospho-MLKL (Ser345) Antibody, clone 7C6.1

品牌
Sigma-Aldrich
货号
MABC1158
规格纯度
clone 7C6.1, from mouse
价格
4324.87 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-phospho-MLKL (Ser345) Antibody, clone 7C6.1
Mixed lineage kinase domain-like protein
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Mixed lineage kinase domain-like protein (UniProt: Q9D2Y4; also known as MLKL) is encoded by the Mlkl gene (Gene ID: 74568) in murine species. MLKL is a pseudokinase that plays a key role in TNF-induced necroptosis. MLKL is highly expressed in thymus, colon, intestine, liver, spleen and lung and is expressed at much lower level in skeletal muscle, heart and kidney. It is not detected in brain. MLKL is activated following phosphorylation by RIPK3 that induces a conformational switch, which is essential for necroptosis. The protein kinase domain of MLKL is catalytically inactive, but contains a pseudoactive site with an interaction between Lys-219 and Gln-343 residues. Upon phosphorylation by RIPK3, MLKL undergoes an active conformation and homotrimerization through its amino-terminal coiled-coil domain. Phosphorylation is also essential for homotrimerization of MLKL and its subsequent localization to the plasma membrane. Plasma membrane localization of MLKL is considered to be essential for Ca2+ influx, which is an early event of TNF-induced necroptosis. (Ref.: Cai, Z et al. (2014). Nat. Cell Biol. 16(1): 55-65).

特异性

Clone 7C6.1 specifically detects MLKL phosphorylalated on Ser345. It targets a sequence of 19 amino acids surrounding Ser345 in the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 19 amino acids surrounding Ser345 from the C-terminal half of murine Mixed lineage kinase domain-like protein (MLKL).

应用

ELISA Analysis: A representative lot detected phospho-MLKL (Ser345) in ELISA applications (Rodriguez, D.A., et. al. (2016). 23(1):76-88).

Immunocytochemistry Analysis: A representative lot detected phospho-MLKL (Ser345) in Immunocytochemistry applications (Rodriguez, D.A., et. al. (2016). 23(1):76-88).

Western Blotting Analysis: A representative lot detected phospho-MLKL (Ser345) in Western Blotting applications (Rodriguez, D.A., et. al. (2016). 23(1):76-88).

Detect phospho MLKL Ser345 using this mouse monoclonal Anti-phospho-MLKL (Ser345), clone 7C6.1, Cat. No. MABC1158. It has been tested in ELISA, Immunocytochemistry, and Western Blotting.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in MLKL -/- mouse embryonic fibroblasts expressing doxycycline-inducible wild-type MLKL and treated with TNF alpha and Z-VAD-FMK.

Western Blotting Analysis: A 1:4,000 dilution of this antibody detected phospho-MLKL Ser345 in 20 ug of lysate from MLKL -/- mouse embryonic fibroblasts expressing doxycycline-inducible wild-type MLKL-Flag-C construct and treated with TNF alpha (10 ng/mL) and Z-VAD-FMK (25 uM).


目标描述

~54 kDa observed; 54.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 7C6.1, monoclonal
species reactivity mouse
technique(s) ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型 IgG2bκ
NCBI登记号 NP_001297542
UniProt登记号 Q9D2Y4
运输 ambient

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map