Anti-PARG Antibody, clone D8B10

半岛bd体育手机客户端 说明

一般描述

Poly(ADP-ribose) glycohydrolase (PARG) is an enzyme possessing both endo- and exoglycosidase activity against poly (ADP-ribose) (PARP), rapidly degrading PARP to release large quantities of free ADP-ribose. PARG is involved in several cellular processes including; apoptosis, DNA repair, cell cycle progression, cell survival and cellular differentiation. During apoptosis, PARG is cleaved by caspase-3 suggesting that PARG activity is regulated during this process. Although encoded by one gene, PARG is present in different cellular localizations as different isoforms: Isoform 1 (111 kDa) is present in the nucleus, Isoform 2 (102 kDA) is in the cytoplasm and Isoform 3 (99 kDa) is mitochondrial. Studies have indicated a critical role for PARG isoform 1 in the quality of sperm chromatin, and subsequent embryonic survival.

免疫原

Histidine-tagged recombinant protein corresponding to human PARG.
Epitope: Unknown

应用

Anti-PARG Antibody, clone D8B10 is an antibody against PARG for use in WB & IP.
Research Category
Signaling

Epigenetics & Nuclear Function
Research Sub Category
Hormones & Receptors

Chromatin Biology

质量

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected PARG on 10 ?g of Jurkat cell lysate.

目标描述

~ 102 kDa observed. Other isoforms may be observed in some lysates at 111 and 99 kDa

外形

Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Jurkat cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	D8B10, monoclonal
species reactivity	human
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_003622
UniProt登记号	Q86W56
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable