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Anti-mTOR Antibody, clone F11

品牌
Sigma-Aldrich
货号
MABS14
规格纯度
clone F11, from mouse
价格
5426.21 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-mTOR Antibody, clone F11
FK506 binding protein 12-rapamycin associated protein 1, FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FKBP12-rapamycin complex-associated prot
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

免疫原

GST-tagged recombinant protein corresponding to rat mTOR.

应用

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Nemazanyy, I, et al. (2008). Hybridoma, 27:395-398.)
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
This Anti-mTOR Antibody, clone F11 is validated for use in WB, IP for the detection of mTOR.

质量

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected mTOR on 10 ?g of HeLa cell lysate.

目标描述

~ 289 kDa observed

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 F11, monoclonal
species reactivity human
species reactivity (predicted by homology) rat (based on 100% sequence homology)
technique(s) immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_063971
UniProt登记号 P42346
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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